29AJ

Crystal Structure of the human mARC1 M187K variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	60.986, 75.072, 110.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.120 - 1.630
R-factor	0.1889
Rwork	0.188
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (2.0_5936)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.120	1.660
High resolution limit [Å]	1.630	1.630
Rmerge	0.190	1.148
Rmeas	0.198	1.185
Rpim	0.054	0.313
Number of reflections	64043	2850
<I/σ(I)>	8.5	2.2
Completeness [%]	99.9	99.8
Redundancy	13.6
CC(1/2)	0.989	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	100 mM Bis-TRIS propane, 20 mM Na2MoO4, 10 mM TCEP, 27.5 % PEG3350