27XK
A cryo-EM structure of GLP1-Y11-GLP1R-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha | polymer | 361 | 41879.5 | 1 | Bos taurus | ||
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 371 | 40227.0 | 1 | UniProt (P54311) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 3 | C (C) | Glucagon-like peptide 1(7-36) | polymer | 31 | 3362.7 | 1 | UniProt (P01275) | Homo sapiens | GLP-1(7-36) |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) | Bos taurus (domestic cattle) | G gamma-I |
| 5 | E (N) | Nanobody-35 | polymer | 140 | 15343.0 | 1 | Lama glama | ||
| 6 | F (R) | Glucagon-like peptide 1 receptor | polymer | 440 | 50860.8 | 1 | UniProt (P43220) | Homo sapiens (human) | GLP-1 receptor,GLP-1-R,GLP-1R |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
C: 7 - 36 (UniProt: P01275)
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
| Gly 344 | - | expression tag |
| Gly 345 | - | expression tag |
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Ser 348 | - | expression tag |
| Gly 349 | - | expression tag |
| Gly 350 | - | expression tag |
| Gly 351 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Val 356 | - | expression tag |
| Ser 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Trp 359 | - | expression tag |
| Arg 360 | - | expression tag |
| Leu 361 | - | expression tag |
| Phe 362 | - | expression tag |
| Lys 363 | - | expression tag |
| Lys 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Ser 366 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Tyr 11 | Thr 102 | engineered mutation |
| Nh2 37 | - | amidation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 159534.1 | |
| All* | Total formula weight | 159534.1 |
