27IL
Crystal structure of Imine Reductase Mutant(R7) from Actinoalloteichus hymeniacidonis in complex with NADPH
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | 3-hydroxyisobutyrate dehydrogenase-like beta-hydroxyacid dehydrogenase | polymer | 316 | 33152.3 | 2 | UniProt (A0AAC9HQB1) | Actinoalloteichus hymeniacidonis | Beta-hydroxyacid dehydrogenase,3-hydroxyisobutyrate dehydrogenase |
| 2 | C (B) | IMIDAZOLE | non-polymer | 69.1 | 1 | Chemie (IMD) | |||
| 3 | D (A) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 1 | Chemie (NAP) | |||
| 4 | E, F (B, A) | water | water | 18.0 | 246 | Chemie (HOH) |
Sequence modifications
B, A: 1 - 316 (UniProt: A0AAC9HQB1)
| PDB | External Database | Details |
|---|---|---|
| Ser 101 | Asn 101 | engineered mutation |
| Lys 125 | Gly 125 | engineered mutation |
| Phe 129 | Ile 129 | engineered mutation |
| Phe 141 | Leu 141 | engineered mutation |
| Ala 169 | His 169 | engineered mutation |
| Ile 177 | Leu 177 | engineered mutation |
| Ile 187 | Phe 187 | engineered mutation |
| Phe 240 | Ser 240 | engineered mutation |
| His 244 | Met 244 | engineered mutation |
| His 247 | Ala 247 | engineered mutation |
| Asn 249 | Phe 249 | engineered mutation |
| Lys 251 | Asn 251 | engineered mutation |
| Ile 252 | Leu 252 | engineered mutation |
| Ile 273 | Met 273 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 66304.6 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 812.5 | |
| All* | Total formula weight | 67117.1 |
