27IL
Crystal structure of Imine Reductase Mutant(R7) from Actinoalloteichus hymeniacidonis in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2026-05-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.770, 62.820, 95.012 |
| Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
| Resolution | 26.520 - 1.920 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.744 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.1_6048) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.064 | 0.654 |
| Rmeas | 0.078 | 0.841 |
| Rpim | 0.043 | 0.552 |
| Number of reflections | 53657 | 3160 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 98.5 | 86.2 |
| Redundancy | 5.7 | 3.6 |
| CC(1/2) | 0.998 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.2 M imidazole malate, 20% w/v PEG4000 |
