23CU

Cryo-EM structure of the MRGPRX4-miniGsq complex in the apo state, local

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Soluble cytochrome b562,Mas-related G-protein coupled receptor member X4	polymer	472	53396.0	1	UniProt (P0ABE7) UniProt (Q96LA9)	Escherichia coli	Cytochrome b-562,Sensory neuron-specific G-protein coupled receptor 5/6

Sequence modifications

R: -115 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Asp -149	-	expression tag
Tyr -148	-	expression tag
Lys -147	-	expression tag
Asp -146	-	expression tag
Asp -145	-	expression tag
Asp -144	-	expression tag
Asp -143	-	expression tag
Ala -142	-	expression tag
Lys -141	-	expression tag
Leu -140	-	expression tag
Gln -139	-	expression tag
Thr -138	-	expression tag
Met -137	-	expression tag
His -136	-	expression tag
His -135	-	expression tag
His -134	-	expression tag
His -133	-	expression tag
His -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
Glu -126	-	expression tag
Asn -125	-	expression tag
Leu -124	-	expression tag
Tyr -123	-	expression tag
Phe -122	-	expression tag
Gln -121	-	expression tag
Gly -120	-	expression tag
Gly -119	-	expression tag
Thr -118	-	expression tag
Thr -117	-	expression tag
Met -116	-	expression tag
Trp -109	Met 29	conflict
Ile -14	His 124	conflict
Leu -10	-	linker
Gly -9	-	linker
Ser -8	-	linker
Thr -7	-	linker
Leu -6	-	linker
Glu -5	-	linker
Val -4	-	linker
Leu -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker
Pro 1	-	linker

R: 2 - 322 (UniProt: Q96LA9)

PDB	External Database	Details
Ser 83	Leu 83	variant

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	53396.0
All*	Total formula weight	53396.0

*Water molecules are not included.