22EM

Gi bound kappa-opioid receptor in complex with beta01

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	353	40313.9	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40352.1	1	UniProt (P62874) Pfam (PF25391)	Mus musculus (house mouse)	Transducin beta chain 1
3	C (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (domestic cattle)	G gamma-I
4	D (F)	Soluble cytochrome b562,Kappa-type opioid receptor	polymer	624	70171.3	1	UniProt (P0ABE7) UniProt (P41145) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,K-OR-1,KOR-1
5	E (A)	A1E3Y-PHE-LEU-DLY-A1L9X	polymer	5	679.9	1		synthetic construct

Sequence modifications

B: 2 - 354 (UniProt: P63096)

PDB	External Database	Details
Ala 203	Gly 203	conflict
Ser 326	Ala 326	conflict

C: 6 - 340 (UniProt: P62874)

PDB	External Database	Details
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

F: -83 - 21 (UniProt: P0ABE7)

PDB	External Database	Details
Met -107	-	initiating methionine
Lys -106	-	expression tag
Thr -105	-	expression tag
Ile -104	-	expression tag
Ile -103	-	expression tag
Ala -102	-	expression tag
Leu -101	-	expression tag
Ser -100	-	expression tag
Tyr -99	-	expression tag
Ile -98	-	expression tag
Phe -97	-	expression tag
Cys -96	-	expression tag
Leu -95	-	expression tag
Val -94	-	expression tag
Phe -93	-	expression tag
Ala -92	-	expression tag
Asp -91	-	expression tag
Tyr -90	-	expression tag
Lys -89	-	expression tag
Asp -88	-	expression tag
Asp -87	-	expression tag
Asp -86	-	expression tag
Asp -85	-	expression tag
Lys -84	-	expression tag
Trp -77	Met 29	conflict
Ile 18	His 124	conflict
Leu 22	-	linker
Glu 23	-	linker
Asn 24	-	linker
Leu 25	-	linker
Tyr 26	-	linker
Phe 27	-	linker
Gln 28	-	linker
Gly 29	-	linker
Met 30	-	linker
Gly 31	-	linker
Gln 32	-	linker
Pro 33	-	linker
Gly 34	-	linker
Asn 35	-	linker
Gly 36	-	linker
Ser 37	-	linker
Ala 38	-	linker
Phe 39	-	linker
Leu 40	-	linker
Leu 41	-	linker
Ala 42	-	linker
Pro 43	-	linker
Asn 44	-	linker
Arg 45	-	linker
Ser 46	-	linker
His 47	-	linker
Ala 48	-	linker
Pro 49	-	linker
Asp 50	-	linker
His 51	-	linker
Asp 52	-	linker
Val 53	-	linker

F: 54 - 358 (UniProt: P41145)
F: 359 - 516 (PDB: 22EM)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	159378.3
All*	Total formula weight	159378.3

*Water molecules are not included.