21ZX

Crystal structure of Gephyrin E domain with PX-RICS peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Isoform 5 of Gephyrin	polymer	425	46227.0	4	UniProt (Q03555) Pfam (PF03453) Pfam (PF00994) Pfam (PF03454)	Rattus norvegicus (Norway rat)	Putative glycine receptor-tubulin linker protein
2	E, F (F, G)	Rho GTPase-activating protein 32	polymer	48	5440.9	2	UniProt (A7KAX9)	Homo sapiens (human)	Brain-specific Rho GTPase-activating protein,GAB-associated Cdc42/Rac GTPase-activating protein,GC-GAP,GTPase regulator interacting with TrkA,Rho-type GTPase-activating protein 32,Rho/Cdc42/Rac GTPase-activating protein RICS,RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling,p200RhoGAP,p250GAP
3	G, H, J, K, M (A, B, C, D)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL) PubChem (753)
4	I, L (A, C)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT) PubChem (175)
5	N, O, P, Q (A, B, C, D)	water	water		18.0	347	Chemie (HOH)

Sequence modifications

A, B, C, D: 318 - 736 (UniProt: Q03555)

PDB	External Database	Details
Gly 312	-	expression tag
Pro 313	-	expression tag
Gly 314	-	expression tag
Ser 315	-	expression tag
Glu 316	-	expression tag
Phe 317	-	expression tag

F, G: 41 - 82 (UniProt: A7KAX9)

PDB	External Database	Details
Gly 35	-	expression tag
Pro 36	-	expression tag
Gly 37	-	expression tag
Ser 38	-	expression tag
Glu 39	-	expression tag
Phe 40	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	195789.7
Non-Polymers*	Number of molecules	7
	Total formula weight	578.6
All*	Total formula weight	196368.3

*Water molecules are not included.