21LT
Cryo-EM structure of the setmelanotide-bound human melanocortin receptor 4 (MC4R)-Gq complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | Setmelanotide | polymer | 8 | 1081.3 | 1 | synthetic construct | ||
| 2 | B (R) | Melanocortin receptor 4,LgBiT subunit | polymer | 505 | 55571.5 | 1 | UniProt (P32245) Pfam (PF00001) | Homo sapiens (human) | MC4-R |
| 3 | C (A) | G subunit q (Gi1-Gq chimeric) | polymer | 361 | 41724.4 | 1 | Homo sapiens | ||
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT | polymer | 371 | 40227.0 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (domestic cattle) | G gamma-I |
| 6 | F (R) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) PubChem (271) |
Sequence modifications
R: 1 - 332 (UniProt: P32245)
R: 348 - 505 (PDB: 21LT)
B: 2 - 340 (UniProt: P54311)
B: 356 - 366 (PDB: 21LT)
| PDB | External Database | Details |
|---|---|---|
| Gly 333 | - | linker |
| Ser 334 | - | linker |
| Ser 335 | - | linker |
| Gly 336 | - | linker |
| Gly 337 | - | linker |
| Gly 338 | - | linker |
| Gly 339 | - | linker |
| Ser 340 | - | linker |
| Gly 341 | - | linker |
| Gly 342 | - | linker |
| Gly 343 | - | linker |
| Gly 344 | - | linker |
| Ser 345 | - | linker |
| Ser 346 | - | linker |
| Gly 347 | - | linker |
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | linker |
| Ser 342 | - | linker |
| Ser 343 | - | linker |
| Gly 344 | - | linker |
| Gly 345 | - | linker |
| Gly 346 | - | linker |
| Gly 347 | - | linker |
| Ser 348 | - | linker |
| Gly 349 | - | linker |
| Gly 350 | - | linker |
| Gly 351 | - | linker |
| Gly 352 | - | linker |
| Ser 353 | - | linker |
| Ser 354 | - | linker |
| Gly 355 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 146465.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 146505.4 |
