21KT

Wnt3a signalosome extracellular complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, E)	Protein Wnt-3a	polymer	334	37438.3	2	UniProt (P27467)	Mus musculus (house mouse)
2	B, C, F, G (C, D, H, G)	Frizzled-8,Peptidyl-prolyl cis-trans isomerase FKBP1A	polymer	271	29762.5	4	UniProt (Q9H461) UniProt (P62942)	Homo sapiens (human)	Fz-8,hFz8,PPIase FKBP1A,12 kDa FK506-binding protein,12 kDa FKBP,FKBP-12,Calstabin-1,FK506-binding protein 1A,FKBP-1A,Immunophilin FKBP12,Rotamase
3	E, H (F, B)	Low-density lipoprotein receptor-related protein 6,Serine/threonine-protein kinase mTOR	polymer	752	85227.8	2	UniProt (O75581) UniProt (A0A8V8TRG9)	Homo sapiens (human)	LRP-6
4	I, J, K (I, J, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
5	L, M, N, O (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG) PubChem (24139)

Sequence modifications

C, D, H, G: 31 - 155 (UniProt: Q9H461)

PDB	External Database	Details
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
Ala 28	-	expression tag
Ser 29	-	expression tag
Ala 30	-	expression tag
Gly 156	-	linker
Gly 157	-	linker
Gly 158	-	linker
Gly 159	-	linker
Ser 160	-	linker
Gly 161	-	linker
Gly 162	-	linker
Gly 163	-	linker
Gly 164	-	linker
Ser 165	-	linker
Gly 166	-	linker
Gly 167	-	linker
Gly 168	-	linker
Gly 169	-	linker
Ser 170	-	linker
Gly 171	-	linker
Gly 172	-	linker
Gly 173	-	linker
Gly 174	-	linker
Ser 175	-	linker

C, D, H, G: 176 - 282 (UniProt: P62942)

PDB	External Database	Details
Ala 178	Gln 4	engineered mutation
Ala 180	Glu 6	engineered mutation
Ser 197	Cys 23	engineered mutation
Ala 240	Gln 66	engineered mutation
Asp 283	-	expression tag
Tyr 284	-	expression tag
Lys 285	-	expression tag
Asp 286	-	expression tag
Asp 287	-	expression tag
Asp 288	-	expression tag
Asp 289	-	expression tag
Lys 290	-	expression tag

F, B: 630 - 1245 (UniProt: O75581)

PDB	External Database	Details
Asp 622	-	expression tag
Tyr 623	-	expression tag
Lys 624	-	expression tag
Asp 625	-	expression tag
Asp 626	-	expression tag
Asp 627	-	expression tag
Asp 628	-	expression tag
Lys 629	-	expression tag
Gly 1246	-	linker
Gly 1247	-	linker
Gly 1248	-	linker
Gly 1249	-	linker
Ser 1250	-	linker
Gly 1251	-	linker
Gly 1252	-	linker
Gly 1253	-	linker
Gly 1254	-	linker
Ser 1255	-	linker
Gly 1256	-	linker
Gly 1257	-	linker
Gly 1258	-	linker
Gly 1259	-	linker
Ser 1260	-	linker
Gly 1261	-	linker
Gly 1262	-	linker
Gly 1263	-	linker
Gly 1264	-	linker
Ser 1265	-	linker

F, B: 1266 - 1365 (UniProt: A0A8V8TRG9)

PDB	External Database	Details
Ser 1336	Cys 2014	engineered mutation
His 1366	-	expression tag
His 1367	-	expression tag
His 1368	-	expression tag
His 1369	-	expression tag
His 1370	-	expression tag
His 1371	-	expression tag
His 1372	-	expression tag
His 1373	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	364382.1
Branched	Number of molecules	3
	Total formula weight	1273.2
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	366540.2

*Water molecules are not included.