21FY

The cryo-EM structure of IscS-PptA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Cysteine desulfurase IscS	polymer	404	45377.6	2	UniProt (P0A6B7) Pfam (PF00266)	Escherichia coli K-12	NifS protein homolog,ThiI transpersulfidase,TusA transpersulfidase
2	C, D (C, D)	PptA	polymer	270	30272.2	2	UniProt (A0A5Q2WBY0)	Psychrobacter phage vB_PmaS_Y8A
3	E, F (C, D)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)

Sequence modifications

C, D: 1 - 250 (UniProt: A0A5Q2WBY0)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	151299.6
Non-Polymers*	Number of molecules	2
	Total formula weight	703.3
All*	Total formula weight	152002.9

*Water molecules are not included.