21FY

The cryo-EM structure of IscS-PptA complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0002143	biological_process	tRNA wobble position uridine thiolation
A	0002937	biological_process	tRNA 4-thiouridine biosynthesis
A	0003824	molecular_function	catalytic activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0009000	molecular_function	selenocysteine lyase activity
A	0009228	biological_process	thiamine biosynthetic process
A	0009589	biological_process	detection of UV
A	0016226	biological_process	iron-sulfur cluster assembly
A	0016261	biological_process	L-selenocysteine catabolic process
A	0019448	biological_process	L-cysteine catabolic process
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
A	0051537	molecular_function	2 iron, 2 sulfur cluster binding
A	0072348	biological_process	sulfur compound transport
A	0097163	molecular_function	sulfur carrier activity
A	1990221	cellular_component	L-cysteine desulfurase complex
A	1990228	cellular_component	sulfurtransferase complex
A	1990329	cellular_component	IscS-TusA complex
A	1990330	cellular_component	IscS-IscU complex
B	0002143	biological_process	tRNA wobble position uridine thiolation
B	0002937	biological_process	tRNA 4-thiouridine biosynthesis
B	0003824	molecular_function	catalytic activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0009000	molecular_function	selenocysteine lyase activity
B	0009228	biological_process	thiamine biosynthetic process
B	0009589	biological_process	detection of UV
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016261	biological_process	L-selenocysteine catabolic process
B	0019448	biological_process	L-cysteine catabolic process
B	0030170	molecular_function	pyridoxal phosphate binding
B	0031071	molecular_function	cysteine desulfurase activity
B	0044571	biological_process	[2Fe-2S] cluster assembly
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding
B	0072348	biological_process	sulfur compound transport
B	0097163	molecular_function	sulfur carrier activity
B	1990221	cellular_component	L-cysteine desulfurase complex
B	1990228	cellular_component	sulfurtransferase complex
B	1990329	cellular_component	IscS-TusA complex
B	1990330	cellular_component	IscS-IscU complex

Functional Information from PROSITE/UniProt

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. VDLMsfSGHKiygpk.GiGaL

Chain	Residue	Details
A	VAL197-LEU216

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Cysteine persulfide intermediate","evidences":[{"source":"HAMAP-Rule","id":"MF_00331","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12860127","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8663056","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00331","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00331","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12860127","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"description":"via persulfide group","evidences":[{"source":"HAMAP-Rule","id":"MF_00331","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	Modified residue: {"description":"N6-(pyridoxal phosphate)lysine","evidences":[{"source":"HAMAP-Rule","id":"MF_00331","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"12860127","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---