1YO7

Re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Regulatory protein rop	polymer	120	13695.5	2	UniProt (P03051) Pfam (PF01815)	Escherichia coli	RNA one modulator, ROM
2	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	D, E (A, B)	water	water		18.0	21	Chemie (HOH)

Sequence modifications

A, B: 1 - 56 (UniProt: P03051)

PDB	External Database	Details
Ser 55	Arg 55	engineered mutation
Ala 118	-	SEE REMARK 999
Lys 119	-	SEE REMARK 999
Gly 120	-	SEE REMARK 999

A, B: 63 - 87 (UniProt: P03051)

PDB	External Database	Details
Lys 57	-	SEE REMARK 999
Lys 58	-	SEE REMARK 999
Asn 59	-	SEE REMARK 999
Gln 61	-	SEE REMARK 999
Ile 62	-	SEE REMARK 999

A, B: 91 - 117 (UniProt: P03051)

PDB	External Database	Details
Gly 88	-	SEE REMARK 999
Gly 89	-	SEE REMARK 999
Ser 90	-	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27390.9
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	27456.3

*Water molecules are not included.