1YO7
Re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-07-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.607, 52.830, 50.360 |
| Unit cell angles | 90.00, 94.99, 90.00 |
Refinement procedure
| Resolution | 26.400 - 2.800 |
| R-factor | 0.224 |
| Rwork | 0.216 |
| R-free | 0.31000 |
| Structure solution method | SIR and model building |
| Starting model (for MR) | 1rop |
| RMSD bond length | 0.007 |
| RMSD bond angle | 18.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.400 | 2.980 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 5376 | |
| Completeness [%] | 87.9 | 85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 10% PEG 2000, 50mM Tris-HCl pH6.6, 40mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
