1YO7
Re-engineering topology of the homodimeric ROP protein into a single-chain 4-helix bundle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1994-07-04 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.607, 52.830, 50.360 |
Unit cell angles | 90.00, 94.99, 90.00 |
Refinement procedure
Resolution | 26.400 - 2.800 |
R-factor | 0.224 |
Rwork | 0.216 |
R-free | 0.31000 |
Structure solution method | SIR and model building |
Starting model (for MR) | 1rop |
RMSD bond length | 0.007 |
RMSD bond angle | 18.000 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.400 | 2.980 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 5376 | |
Completeness [%] | 87.9 | 85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 10% PEG 2000, 50mM Tris-HCl pH6.6, 40mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |