1XRK

Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bleomycin resistance protein	polymer	124	13919.4	2	UniProt (P17493) Pfam (PF19581)	Streptoalloteichus hindustanus	Bleomycin binding protein, BRP, Phleomycin resistance protein
2	C, E, F (A, B)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
3	D, G (A, B)	BLEOMYCIN A2	non-polymer		1416.6	2	Chemie (BLM)
4	H, I (A, B)	water	water		18.0	288	Chemie (HOH)

Sequence modifications

A, B: 1 - 124 (UniProt: P17493)

PDB	External Database	Details
Glu 18	Gly 18	engineered mutation
Val 32	Asp 32	engineered mutation
Gln 63	Leu 63	engineered mutation
Val 98	Gly 98	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	27838.7
Non-Polymers*	Number of molecules	5
	Total formula weight	3121.3
All*	Total formula weight	30960.0

*Water molecules are not included.