1XRK
Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9083 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.983, 66.617, 47.179 |
| Unit cell angles | 90.00, 117.43, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.500 |
| R-factor | 0.17508 |
| Rwork | 0.174 |
| R-free | 0.19425 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jie |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 36444 | |
| <I/σ(I)> | 20.3 | 3.9 |
| Completeness [%] | 94.4 | 88.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
