1XG3
Crystal structure of the C123S 2-methylisocitrate lyase mutant from Escherichia coli in complex with the reaction product, Mg(II)-pyruvate and succinate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Probable methylisocitrate lyase | polymer | 295 | 32022.4 | 4 | UniProt (P77541) Pfam (PF13714) | Escherichia coli | 2-methylisocitrate lyase |
| 2 | E, H, K, M (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 3 | F, I, L, N (A, B, C, D) | PYRUVIC ACID | non-polymer | 88.1 | 4 | Chemie (PYR) | |||
| 4 | G, J, O (A, B, D) | SUCCINIC ACID | non-polymer | 118.1 | 3 | Chemie (SIN) | |||
| 5 | P, Q, R, S (A, B, C, D) | water | water | 18.0 | 1061 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 296 (UniProt: P77541)
| PDB | External Database | Details |
|---|---|---|
| Ser 123 | Cys 122 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 128089.4 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 803.7 | |
| All* | Total formula weight | 128893.2 |
