1XFX

Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 10 millimolar exogenously added calcium chloride

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Calmodulin-sensitive adenylate cyclase	polymer	777	90128.9	6	UniProt (P40136) Pfam (PF07737) Pfam (PF03497) Pfam (PF20850)	Bacillus anthracis	ATP pyrophosphate-lyase, Adenylyl cyclase, Edema factor, EF, Anthrax edema toxin adenylate cyclase component
2	G, H, I, J, K... (O, P, Q, R, S...)	Calmodulin 2	polymer	149	17320.5	6	UniProt (P62158) Pfam (PF13499) UniProt (by SIFTS) (P0DP23)	Homo sapiens (human)
3	M, N, O, P, Q... (A, B, C, D, E...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
4	AA, BA, CA, DA, EA... (Q, R, S, T, O...)	CALCIUM ION	non-polymer		40.1	24	Chemie (CA)

Sequence modifications

A, B, C, D, E, F: 33 - 800 (UniProt: P40136)

PDB	External Database	Details
Met 24	-	initiating methionine
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag
His 29	-	expression tag
His 30	-	expression tag
Ala 31	-	cloning artifact
Ala 32	-	cloning artifact

O, P, Q, R, S, T: 0 - 148 (UniProt: P62158)

PDB	External Database	Details
Mse 0	Met 1	modified residue
Mse 36	Met 37	modified residue
Mse 51	Met 52	modified residue
Mse 71	Met 72	modified residue
Mse 72	Met 73	modified residue
Mse 76	Met 77	modified residue
Mse 109	Met 110	modified residue
Mse 124	Met 125	modified residue
Mse 144	Met 145	modified residue
Mse 145	Met 146	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	644696.5
Non-Polymers*	Number of molecules	30
	Total formula weight	1107.7
All*	Total formula weight	645804.2

*Water molecules are not included.