1XFX

Crystal structure of anthrax edema factor (EF) in complex with calmodulin in the presence of 10 millimolar exogenously added calcium chloride

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005576	cellular_component	extracellular region
A	0008237	molecular_function	metallopeptidase activity
A	0008294	molecular_function	calcium- and calmodulin-responsive adenylate cyclase activity
A	0046872	molecular_function	metal ion binding
B	0003824	molecular_function	catalytic activity
B	0005576	cellular_component	extracellular region
B	0008237	molecular_function	metallopeptidase activity
B	0008294	molecular_function	calcium- and calmodulin-responsive adenylate cyclase activity
B	0046872	molecular_function	metal ion binding
C	0003824	molecular_function	catalytic activity
C	0005576	cellular_component	extracellular region
C	0008237	molecular_function	metallopeptidase activity
C	0008294	molecular_function	calcium- and calmodulin-responsive adenylate cyclase activity
C	0046872	molecular_function	metal ion binding
D	0003824	molecular_function	catalytic activity
D	0005576	cellular_component	extracellular region
D	0008237	molecular_function	metallopeptidase activity
D	0008294	molecular_function	calcium- and calmodulin-responsive adenylate cyclase activity
D	0046872	molecular_function	metal ion binding
E	0003824	molecular_function	catalytic activity
E	0005576	cellular_component	extracellular region
E	0008237	molecular_function	metallopeptidase activity
E	0008294	molecular_function	calcium- and calmodulin-responsive adenylate cyclase activity
E	0046872	molecular_function	metal ion binding
F	0003824	molecular_function	catalytic activity
F	0005576	cellular_component	extracellular region
F	0008237	molecular_function	metallopeptidase activity
F	0008294	molecular_function	calcium- and calmodulin-responsive adenylate cyclase activity
F	0046872	molecular_function	metal ion binding
O	0000086	biological_process	G2/M transition of mitotic cell cycle
O	0000922	cellular_component	spindle pole
O	0002027	biological_process	regulation of heart rate
O	0005246	molecular_function	calcium channel regulator activity
O	0005509	molecular_function	calcium ion binding
O	0005513	biological_process	detection of calcium ion
O	0005515	molecular_function	protein binding
O	0005576	cellular_component	extracellular region
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005813	cellular_component	centrosome
O	0005819	cellular_component	spindle
O	0005829	cellular_component	cytosol
O	0005876	cellular_component	spindle microtubule
O	0005886	cellular_component	plasma membrane
O	0007186	biological_process	G protein-coupled receptor signaling pathway
O	0008076	cellular_component	voltage-gated potassium channel complex
O	0010856	molecular_function	adenylate cyclase activator activity
O	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
O	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
O	0016020	cellular_component	membrane
O	0016240	biological_process	autophagosome membrane docking
O	0019855	molecular_function	calcium channel inhibitor activity
O	0019901	molecular_function	protein kinase binding
O	0021762	biological_process	substantia nigra development
O	0030017	cellular_component	sarcomere
O	0030672	cellular_component	synaptic vesicle membrane
O	0031432	molecular_function	titin binding
O	0031514	cellular_component	motile cilium
O	0031982	cellular_component	vesicle
O	0032465	biological_process	regulation of cytokinesis
O	0032991	cellular_component	protein-containing complex
O	0034704	cellular_component	calcium channel complex
O	0035458	biological_process	cellular response to interferon-beta
O	0043209	cellular_component	myelin sheath
O	0043539	molecular_function	protein serine/threonine kinase activator activity
O	0044305	cellular_component	calyx of Held
O	0044325	molecular_function	transmembrane transporter binding
O	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
O	0046872	molecular_function	metal ion binding
O	0048306	molecular_function	calcium-dependent protein binding
O	0050848	biological_process	regulation of calcium-mediated signaling
O	0051592	biological_process	response to calcium ion
O	0055117	biological_process	regulation of cardiac muscle contraction
O	0060291	biological_process	long-term synaptic potentiation
O	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
O	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
O	0071346	biological_process	cellular response to type II interferon
O	0072542	molecular_function	protein phosphatase activator activity
O	0097225	cellular_component	sperm midpiece
O	0097720	biological_process	calcineurin-mediated signaling
O	0098901	biological_process	regulation of cardiac muscle cell action potential
O	0099523	cellular_component	presynaptic cytosol
O	0140056	biological_process	organelle localization by membrane tethering
O	0140238	biological_process	presynaptic endocytosis
O	0141110	molecular_function	transporter inhibitor activity
O	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
O	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
O	1902494	cellular_component	catalytic complex
O	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
O	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
P	0000086	biological_process	G2/M transition of mitotic cell cycle
P	0000922	cellular_component	spindle pole
P	0002027	biological_process	regulation of heart rate
P	0005246	molecular_function	calcium channel regulator activity
P	0005509	molecular_function	calcium ion binding
P	0005513	biological_process	detection of calcium ion
P	0005515	molecular_function	protein binding
P	0005576	cellular_component	extracellular region
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005813	cellular_component	centrosome
P	0005819	cellular_component	spindle
P	0005829	cellular_component	cytosol
P	0005876	cellular_component	spindle microtubule
P	0005886	cellular_component	plasma membrane
P	0007186	biological_process	G protein-coupled receptor signaling pathway
P	0008076	cellular_component	voltage-gated potassium channel complex
P	0010856	molecular_function	adenylate cyclase activator activity
P	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
P	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
P	0016020	cellular_component	membrane
P	0016240	biological_process	autophagosome membrane docking
P	0019855	molecular_function	calcium channel inhibitor activity
P	0019901	molecular_function	protein kinase binding
P	0021762	biological_process	substantia nigra development
P	0030017	cellular_component	sarcomere
P	0030672	cellular_component	synaptic vesicle membrane
P	0031432	molecular_function	titin binding
P	0031514	cellular_component	motile cilium
P	0031982	cellular_component	vesicle
P	0032465	biological_process	regulation of cytokinesis
P	0032991	cellular_component	protein-containing complex
P	0034704	cellular_component	calcium channel complex
P	0035458	biological_process	cellular response to interferon-beta
P	0043209	cellular_component	myelin sheath
P	0043539	molecular_function	protein serine/threonine kinase activator activity
P	0044305	cellular_component	calyx of Held
P	0044325	molecular_function	transmembrane transporter binding
P	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
P	0046872	molecular_function	metal ion binding
P	0048306	molecular_function	calcium-dependent protein binding
P	0050848	biological_process	regulation of calcium-mediated signaling
P	0051592	biological_process	response to calcium ion
P	0055117	biological_process	regulation of cardiac muscle contraction
P	0060291	biological_process	long-term synaptic potentiation
P	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
P	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
P	0071346	biological_process	cellular response to type II interferon
P	0072542	molecular_function	protein phosphatase activator activity
P	0097225	cellular_component	sperm midpiece
P	0097720	biological_process	calcineurin-mediated signaling
P	0098901	biological_process	regulation of cardiac muscle cell action potential
P	0099523	cellular_component	presynaptic cytosol
P	0140056	biological_process	organelle localization by membrane tethering
P	0140238	biological_process	presynaptic endocytosis
P	0141110	molecular_function	transporter inhibitor activity
P	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
P	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
P	1902494	cellular_component	catalytic complex
P	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
P	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
Q	0000086	biological_process	G2/M transition of mitotic cell cycle
Q	0000922	cellular_component	spindle pole
Q	0002027	biological_process	regulation of heart rate
Q	0005246	molecular_function	calcium channel regulator activity
Q	0005509	molecular_function	calcium ion binding
Q	0005513	biological_process	detection of calcium ion
Q	0005515	molecular_function	protein binding
Q	0005576	cellular_component	extracellular region
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005813	cellular_component	centrosome
Q	0005819	cellular_component	spindle
Q	0005829	cellular_component	cytosol
Q	0005876	cellular_component	spindle microtubule
Q	0005886	cellular_component	plasma membrane
Q	0007186	biological_process	G protein-coupled receptor signaling pathway
Q	0008076	cellular_component	voltage-gated potassium channel complex
Q	0010856	molecular_function	adenylate cyclase activator activity
Q	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
Q	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
Q	0016020	cellular_component	membrane
Q	0016240	biological_process	autophagosome membrane docking
Q	0019855	molecular_function	calcium channel inhibitor activity
Q	0019901	molecular_function	protein kinase binding
Q	0021762	biological_process	substantia nigra development
Q	0030017	cellular_component	sarcomere
Q	0030672	cellular_component	synaptic vesicle membrane
Q	0031432	molecular_function	titin binding
Q	0031514	cellular_component	motile cilium
Q	0031982	cellular_component	vesicle
Q	0032465	biological_process	regulation of cytokinesis
Q	0032991	cellular_component	protein-containing complex
Q	0034704	cellular_component	calcium channel complex
Q	0035458	biological_process	cellular response to interferon-beta
Q	0043209	cellular_component	myelin sheath
Q	0043539	molecular_function	protein serine/threonine kinase activator activity
Q	0044305	cellular_component	calyx of Held
Q	0044325	molecular_function	transmembrane transporter binding
Q	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
Q	0046872	molecular_function	metal ion binding
Q	0048306	molecular_function	calcium-dependent protein binding
Q	0050848	biological_process	regulation of calcium-mediated signaling
Q	0051592	biological_process	response to calcium ion
Q	0055117	biological_process	regulation of cardiac muscle contraction
Q	0060291	biological_process	long-term synaptic potentiation
Q	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
Q	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
Q	0071346	biological_process	cellular response to type II interferon
Q	0072542	molecular_function	protein phosphatase activator activity
Q	0097225	cellular_component	sperm midpiece
Q	0097720	biological_process	calcineurin-mediated signaling
Q	0098901	biological_process	regulation of cardiac muscle cell action potential
Q	0099523	cellular_component	presynaptic cytosol
Q	0140056	biological_process	organelle localization by membrane tethering
Q	0140238	biological_process	presynaptic endocytosis
Q	0141110	molecular_function	transporter inhibitor activity
Q	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
Q	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
Q	1902494	cellular_component	catalytic complex
Q	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
Q	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
R	0000086	biological_process	G2/M transition of mitotic cell cycle
R	0000922	cellular_component	spindle pole
R	0002027	biological_process	regulation of heart rate
R	0005246	molecular_function	calcium channel regulator activity
R	0005509	molecular_function	calcium ion binding
R	0005513	biological_process	detection of calcium ion
R	0005515	molecular_function	protein binding
R	0005576	cellular_component	extracellular region
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005813	cellular_component	centrosome
R	0005819	cellular_component	spindle
R	0005829	cellular_component	cytosol
R	0005876	cellular_component	spindle microtubule
R	0005886	cellular_component	plasma membrane
R	0007186	biological_process	G protein-coupled receptor signaling pathway
R	0008076	cellular_component	voltage-gated potassium channel complex
R	0010856	molecular_function	adenylate cyclase activator activity
R	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
R	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
R	0016020	cellular_component	membrane
R	0016240	biological_process	autophagosome membrane docking
R	0019855	molecular_function	calcium channel inhibitor activity
R	0019901	molecular_function	protein kinase binding
R	0021762	biological_process	substantia nigra development
R	0030017	cellular_component	sarcomere
R	0030672	cellular_component	synaptic vesicle membrane
R	0031432	molecular_function	titin binding
R	0031514	cellular_component	motile cilium
R	0031982	cellular_component	vesicle
R	0032465	biological_process	regulation of cytokinesis
R	0032991	cellular_component	protein-containing complex
R	0034704	cellular_component	calcium channel complex
R	0035458	biological_process	cellular response to interferon-beta
R	0043209	cellular_component	myelin sheath
R	0043539	molecular_function	protein serine/threonine kinase activator activity
R	0044305	cellular_component	calyx of Held
R	0044325	molecular_function	transmembrane transporter binding
R	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
R	0046872	molecular_function	metal ion binding
R	0048306	molecular_function	calcium-dependent protein binding
R	0050848	biological_process	regulation of calcium-mediated signaling
R	0051592	biological_process	response to calcium ion
R	0055117	biological_process	regulation of cardiac muscle contraction
R	0060291	biological_process	long-term synaptic potentiation
R	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
R	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
R	0071346	biological_process	cellular response to type II interferon
R	0072542	molecular_function	protein phosphatase activator activity
R	0097225	cellular_component	sperm midpiece
R	0097720	biological_process	calcineurin-mediated signaling
R	0098901	biological_process	regulation of cardiac muscle cell action potential
R	0099523	cellular_component	presynaptic cytosol
R	0140056	biological_process	organelle localization by membrane tethering
R	0140238	biological_process	presynaptic endocytosis
R	0141110	molecular_function	transporter inhibitor activity
R	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
R	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
R	1902494	cellular_component	catalytic complex
R	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
R	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
S	0000086	biological_process	G2/M transition of mitotic cell cycle
S	0000922	cellular_component	spindle pole
S	0002027	biological_process	regulation of heart rate
S	0005246	molecular_function	calcium channel regulator activity
S	0005509	molecular_function	calcium ion binding
S	0005513	biological_process	detection of calcium ion
S	0005515	molecular_function	protein binding
S	0005576	cellular_component	extracellular region
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005819	cellular_component	spindle
S	0005829	cellular_component	cytosol
S	0005876	cellular_component	spindle microtubule
S	0005886	cellular_component	plasma membrane
S	0007186	biological_process	G protein-coupled receptor signaling pathway
S	0008076	cellular_component	voltage-gated potassium channel complex
S	0010856	molecular_function	adenylate cyclase activator activity
S	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
S	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
S	0016020	cellular_component	membrane
S	0016240	biological_process	autophagosome membrane docking
S	0019855	molecular_function	calcium channel inhibitor activity
S	0019901	molecular_function	protein kinase binding
S	0021762	biological_process	substantia nigra development
S	0030017	cellular_component	sarcomere
S	0030672	cellular_component	synaptic vesicle membrane
S	0031432	molecular_function	titin binding
S	0031514	cellular_component	motile cilium
S	0031982	cellular_component	vesicle
S	0032465	biological_process	regulation of cytokinesis
S	0032991	cellular_component	protein-containing complex
S	0034704	cellular_component	calcium channel complex
S	0035458	biological_process	cellular response to interferon-beta
S	0043209	cellular_component	myelin sheath
S	0043539	molecular_function	protein serine/threonine kinase activator activity
S	0044305	cellular_component	calyx of Held
S	0044325	molecular_function	transmembrane transporter binding
S	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
S	0046872	molecular_function	metal ion binding
S	0048306	molecular_function	calcium-dependent protein binding
S	0050848	biological_process	regulation of calcium-mediated signaling
S	0051592	biological_process	response to calcium ion
S	0055117	biological_process	regulation of cardiac muscle contraction
S	0060291	biological_process	long-term synaptic potentiation
S	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
S	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
S	0071346	biological_process	cellular response to type II interferon
S	0072542	molecular_function	protein phosphatase activator activity
S	0097225	cellular_component	sperm midpiece
S	0097720	biological_process	calcineurin-mediated signaling
S	0098901	biological_process	regulation of cardiac muscle cell action potential
S	0099523	cellular_component	presynaptic cytosol
S	0140056	biological_process	organelle localization by membrane tethering
S	0140238	biological_process	presynaptic endocytosis
S	0141110	molecular_function	transporter inhibitor activity
S	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
S	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
S	1902494	cellular_component	catalytic complex
S	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
S	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering
T	0000086	biological_process	G2/M transition of mitotic cell cycle
T	0000922	cellular_component	spindle pole
T	0002027	biological_process	regulation of heart rate
T	0005246	molecular_function	calcium channel regulator activity
T	0005509	molecular_function	calcium ion binding
T	0005513	biological_process	detection of calcium ion
T	0005515	molecular_function	protein binding
T	0005576	cellular_component	extracellular region
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005813	cellular_component	centrosome
T	0005819	cellular_component	spindle
T	0005829	cellular_component	cytosol
T	0005876	cellular_component	spindle microtubule
T	0005886	cellular_component	plasma membrane
T	0007186	biological_process	G protein-coupled receptor signaling pathway
T	0008076	cellular_component	voltage-gated potassium channel complex
T	0010856	molecular_function	adenylate cyclase activator activity
T	0010880	biological_process	regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
T	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
T	0016020	cellular_component	membrane
T	0016240	biological_process	autophagosome membrane docking
T	0019855	molecular_function	calcium channel inhibitor activity
T	0019901	molecular_function	protein kinase binding
T	0021762	biological_process	substantia nigra development
T	0030017	cellular_component	sarcomere
T	0030672	cellular_component	synaptic vesicle membrane
T	0031432	molecular_function	titin binding
T	0031514	cellular_component	motile cilium
T	0031982	cellular_component	vesicle
T	0032465	biological_process	regulation of cytokinesis
T	0032991	cellular_component	protein-containing complex
T	0034704	cellular_component	calcium channel complex
T	0035458	biological_process	cellular response to interferon-beta
T	0043209	cellular_component	myelin sheath
T	0043539	molecular_function	protein serine/threonine kinase activator activity
T	0044305	cellular_component	calyx of Held
T	0044325	molecular_function	transmembrane transporter binding
T	0046427	biological_process	positive regulation of receptor signaling pathway via JAK-STAT
T	0046872	molecular_function	metal ion binding
T	0048306	molecular_function	calcium-dependent protein binding
T	0050848	biological_process	regulation of calcium-mediated signaling
T	0051592	biological_process	response to calcium ion
T	0055117	biological_process	regulation of cardiac muscle contraction
T	0060291	biological_process	long-term synaptic potentiation
T	0060314	biological_process	regulation of ryanodine-sensitive calcium-release channel activity
T	0060315	biological_process	negative regulation of ryanodine-sensitive calcium-release channel activity
T	0071346	biological_process	cellular response to type II interferon
T	0072542	molecular_function	protein phosphatase activator activity
T	0097225	cellular_component	sperm midpiece
T	0097720	biological_process	calcineurin-mediated signaling
T	0098901	biological_process	regulation of cardiac muscle cell action potential
T	0099523	cellular_component	presynaptic cytosol
T	0140056	biological_process	organelle localization by membrane tethering
T	0140238	biological_process	presynaptic endocytosis
T	0141110	molecular_function	transporter inhibitor activity
T	1901842	biological_process	negative regulation of high voltage-gated calcium channel activity
T	1901844	biological_process	regulation of cell communication by electrical coupling involved in cardiac conduction
T	1902494	cellular_component	catalytic complex
T	1905913	biological_process	negative regulation of calcium ion export across plasma membrane
T	1990456	biological_process	mitochondrion-endoplasmic reticulum membrane tethering

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 900

Chain	Residue
A	ASP491
A	ASP493
A	HIS577

---

site_id	AC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG B 901

Chain	Residue
B	ASP491
B	ASP493
B	HIS577

---

site_id	AC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG C 902

Chain	Residue
C	ASP491
C	ASP493
C	HIS577

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 903

Chain	Residue
D	ASP491
D	ASP493
D	HIS577

---

site_id	AC5
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG E 904

Chain	Residue
E	ASP491
E	ASP493
E	HIS577

---

site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG F 905

Chain	Residue
F	ASP491
F	ASP493
F	HIS577

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA O 701

Chain	Residue
O	ASP20
O	ASP22
O	ASP24
O	THR26

---

site_id	AC8
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA O 702

Chain	Residue
O	VAL55
O	ASP56
O	ALA57
O	ASP58
O	GLY59
O	GLY61
O	THR62

---

site_id	AC9
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA P 703

Chain	Residue
P	ASP20
P	ASP22
P	ASP24
P	THR26

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA P 704

Chain	Residue
P	VAL55
P	ASP56
P	ALA57
P	ASP58
P	GLY59
P	GLY61
P	THR62

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA Q 705

Chain	Residue
Q	ASP20
Q	ASP22
Q	ASP24
Q	THR26

---

site_id	BC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA Q 706

Chain	Residue
Q	VAL55
Q	ASP56
Q	ALA57
Q	ASP58
Q	GLY59
Q	GLY61
Q	THR62

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA R 707

Chain	Residue
R	ASP20
R	ASP22
R	ASP24
R	THR26

---

site_id	BC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA R 708

Chain	Residue
R	VAL55
R	ASP56
R	ALA57
R	ASP58
R	GLY59
R	GLY61
R	THR62

---

site_id	BC6
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA S 709

Chain	Residue
S	ASP20
S	ASP22
S	ASP24
S	THR26

---

site_id	BC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA S 710

Chain	Residue
S	VAL55
S	ASP56
S	ALA57
S	ASP58
S	GLY59
S	GLY61
S	THR62

---

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CA T 711

Chain	Residue
T	ASP20
T	ASP22
T	ASP24
T	THR26

---

site_id	BC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE CA T 712

Chain	Residue
T	VAL55
T	ASP56
T	ALA57
T	ASP58
T	GLY59
T	GLY61
T	THR62

---

site_id	CC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA O 801

Chain	Residue
O	ASP129
O	ASP131
O	ASP133
O	GLN135
O	GLU140

---

site_id	CC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA O 802

Chain	Residue
O	ASP93
O	ASP95
O	ASN97
O	TYR99
O	GLU104

---

site_id	CC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA P 803

Chain	Residue
P	ASP129
P	ASP131
P	ASP133
P	GLN135
P	GLU140

---

site_id	CC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA P 804

Chain	Residue
P	ASP93
P	ASP95
P	ASN97
P	TYR99
P	GLU104

---

site_id	CC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA Q 805

Chain	Residue
Q	ASP129
Q	ASP131
Q	ASP133
Q	GLN135
Q	GLU140

---

site_id	CC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA Q 806

Chain	Residue
Q	ASP93
Q	ASP95
Q	ASN97
Q	TYR99
Q	GLU104

---

site_id	CC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA R 807

Chain	Residue
R	ASP129
R	ASP131
R	ASP133
R	GLN135
R	GLU140

---

site_id	CC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA R 808

Chain	Residue
R	ASP93
R	ASP95
R	ASN97
R	TYR99
R	GLU104

---

site_id	CC9
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA S 809

Chain	Residue
S	ASP129
S	ASP131
S	ASP133
S	GLN135
S	GLU140

---

site_id	DC1
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA S 810

Chain	Residue
S	ASP93
S	ASP95
S	ASN97
S	TYR99
S	GLU104

---

site_id	DC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA T 811

Chain	Residue
T	ASP129
T	ASP131
T	ASP133
T	GLN135
T	GLU140

---

site_id	DC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CA T 812

Chain	Residue
T	ASP93
T	ASP95
T	ASN97
T	TYR99
T	GLU104

---

Functional Information from PROSITE/UniProt

site_id	PS00018
Number of Residues	13
Details	EF_HAND_1 EF-hand calcium-binding domain. DKDGDGTITtkEL

Chain	Residue	Details
O	ASP20-LEU32
O	ASP56-PHE68
O	ASP93-LEU105
O	ASP129-PHE141

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	330
Details	Region: {"description":"Catalytic CA1","evidences":[{"source":"PubMed","id":"11807546","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	834
Details	Region: {"description":"Catalytic CB","evidences":[{"source":"PubMed","id":"11807546","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	792
Details	Region: {"description":"Catalytic CA2","evidences":[{"source":"PubMed","id":"11807546","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"11807546","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15719022","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1XFU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1XFV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1XFW","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1XFX","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1XFY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1XFZ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1Y0V","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	18
Details	Binding site: {"evidences":[{"source":"PubMed","id":"15719022","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1XFW","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	60
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1474585","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25441029","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27564677","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1CLL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4UMO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4V0C","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5J03","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	30
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00448","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"1474585","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"27564677","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"29724949","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1CLL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"5J03","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6CNN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6CNO","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	6
Details	Modified residue: {"description":"N-acetylalanine","evidences":[{"source":"PubMed","id":"7093203","evidenceCode":"ECO:0000269"},{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Bensaad K.","Vousden K.H."]}},{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22223895","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"22814378","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"25944712","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	6
Details	Modified residue: {"description":"Phosphothreonine; by CaMK4","evidences":[{"source":"UniProtKB","id":"P0DP29","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	6
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	6
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	6
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	6
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	6
Details	Modified residue: {"description":"N6-methyllysine; alternate","evidences":[{"source":"PubMed","id":"24129315","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	12
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"UniProtKB","id":"P62157","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---