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1X9S

T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-CTP as the incoming nucleotide.

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1P5'-D(*GP*GP*AP*GP*AP*GP*TP*GP*AP*TP*T*GP*GP*TP*AP*GP*TP*GP*TP*GP*AP*(2DT))-3'polymer226926.51
2T5'-D(*CP*CP*CP*(AFG)P*AP*TP*CP*AP*CP*AP*CP*TP*AP*CP*CP*AP*AP*TP*CP*AP*CP*TP*CP*TP*CP*C)-3'polymer267936.21
3ADNA polymerasepolymer69879089.81UniProt (P00581)
Pfam (PF00476)
In PDB
Enterobacteria phage T7T7 DNA polymerase
4BThioredoxin 1polymer10811687.41UniProt (P0AA25)
Pfam (PF00085)
In PDB
Escherichia coliTRX1, TRX
5AMAGNESIUM IONnon-polymer24.31Chemie (MG)
6waterwater18.051Chemie (HOH)
Sequence modifications
A: 1 - 704 (UniProt: P00581)
PDBExternal DatabaseDetails
-Lys 118deletion
-Arg 119deletion
-Phe 120deletion
-Gly 121deletion
-Ser 122deletion
-His 123deletion
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight105639.9
Non-Polymers*Number of molecules1
Total formula weight24.3
All*Total formula weight105664.2
*Water molecules are not included.

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PDB entries from 2024-09-18

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