1X9S
T7 DNA polymerase in complex with a primer/template DNA containing a disordered N-2 aminofluorene on the template, crystallized with dideoxy-CTP as the incoming nucleotide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.91600 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.579, 211.103, 51.991 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.640 - 2.700 |
R-factor | 0.229 |
Rwork | 0.226 |
R-free | 0.27900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 33030 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 8000, ammonium sulfate, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 8000 | ||
2 | 1 | 1 | ammonium sulfate | ||
3 | 1 | 1 | magnesium chloride | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 8000 | ||
6 | 1 | 2 | ammonium sulfate | ||
7 | 1 | 2 | magnesium chloride | ||
8 | 1 | 2 | H2O |