1WSE

Co-crystal structure of E.coli RNase HI active site mutant (E48A*) with Mn2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ribonuclease HI	polymer	155	17506.9	2	UniProt (P0A7Y4) Pfam (PF00075)	Escherichia coli	RNase HI, Ribonuclease H, RNase H
2	C, D (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	E, F (A, B)	water	water		18.0	141	Chemie (HOH)

Sequence modifications

A, B: 1 - 155 (UniProt: P0A7Y4)

PDB	External Database	Details
Ala 48	Glu 48	engineered mutation
Ala 87	Lys 87	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	35013.7
Non-Polymers*	Number of molecules	2
	Total formula weight	109.9
All*	Total formula weight	35123.6

*Water molecules are not included.