1VEP
Crystal Structure Analysis of Triple (T47M/Y164E/T328N)/maltose of Bacillus cereus Beta-Amylase at pH 6.5
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Beta-amylase | polymer | 516 | 58367.6 | 1 | UniProt (P36924) Pfam (PF01373) Pfam (PF00686) In PDB | Bacillus cereus | 1,4-alpha-D-glucan maltohydrolase, Glycoside Hydrolase Family 14 |
| 2 | B | alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900018) GlyTouCan (G66120GK) | beta-maltose | ||
| 3 | C, D, E | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 3 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 5 | water | water | 18.0 | 271 | Chemie (HOH) |
Sequence modifications
A: 1 - 516 (UniProt: P36924)
| PDB | External Database | Details |
|---|---|---|
| Met 47 | Thr 77 | engineered mutation |
| Glu 164 | Tyr 194 | engineered mutation |
| Asn 328 | Thr 358 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 58367.6 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1369.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 59776.8 |
