1VEP
Crystal Structure Analysis of Triple (T47M/Y164E/T328N)/maltose of Bacillus cereus Beta-Amylase at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-08-04 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.450, 92.695, 65.755 |
| Unit cell angles | 90.00, 102.37, 90.00 |
Refinement procedure
| Resolution | 14.900 - 2.060 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.840 |
| Number of reflections | 53224 | |
| Completeness [%] | 90.1 | 36.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 6000, ammonium sulfate, potassium phosphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
