1U0C

Y33C Mutant of Homing endonuclease I-CreI

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	5'-D(*GP*CP*TP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*AP*CP*G)-3'	polymer	24	7418.8	1
2	B (D)	5'-D(*CP*GP*TP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*AP*GP*C)-3'	polymer	24	7320.7	1
3	C, D (A, B)	DNA endonuclease I-CreI	polymer	163	18663.5	2	UniProt (P05725) Pfam (PF00961)	Chlamydomonas reinhardtii	23S rRNA intron protein
4	E, F, G (C, A, B)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
5	H, I, J, K (C, D, A, B)	water	water		18.0	90	Chemie (HOH)

Sequence modifications

A, B: 1 - 163 (UniProt: P05725)

PDB	External Database	Details
Cys 33	Tyr 33	engineered mutation
Thr 42	Ala 42	engineered mutation
Glu 47	Gln 47	engineered mutation
Glu 110	Trp 110	engineered mutation
Gln 111	Arg 111	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	52066.6
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	52139.5

*Water molecules are not included.