1U0C
Y33C Mutant of Homing endonuclease I-CreI
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | C | 5'-D(*GP*CP*TP*AP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*AP*CP*G)-3' | polymer | 24 | 7418.8 | 1 | |||
2 | D | 5'-D(*CP*GP*TP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*TP*AP*GP*C)-3' | polymer | 24 | 7320.7 | 1 | |||
3 | A, B | DNA endonuclease I-CreI | polymer | 163 | 18663.5 | 2 | UniProt (P05725) Pfam (PF00961) In PDB | Chlamydomonas reinhardtii | 23S rRNA intron protein |
4 | C, A, B | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
5 | water | water | 18.0 | 90 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 163 (UniProt: P05725)
PDB | External Database | Details |
---|---|---|
Cys 33 | Tyr 33 | engineered mutation |
Thr 42 | Ala 42 | engineered mutation |
Glu 47 | Gln 47 | engineered mutation |
Glu 110 | Trp 110 | engineered mutation |
Gln 111 | Arg 111 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 52066.6 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 72.9 | |
All* | Total formula weight | 52139.5 |