1U0C

Y33C Mutant of Homing endonuclease I-CreI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Collection date	2003-03-26
Spacegroup name	P 1 21 1
Unit cell lengths	43.089, 68.021, 87.182
Unit cell angles	90.00, 92.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.22
Rwork	0.214
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.200
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	17529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1