1TT5
Structure of APPBP1-UBA3-Ubc12N26: a unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | amyloid protein-binding protein 1 | polymer | 531 | 59973.8 | 2 | GenBank (4502169) Pfam (PF00899) UniProt (by SIFTS) (Q13564) | Homo sapiens (human) | APPBP1 |
| 2 | B, D (B, D) | ubiquitin-activating enzyme E1C isoform 1 | polymer | 434 | 48799.8 | 2 | GenBank (38045942) Pfam (PF00899) Pfam (PF08825) UniProt (by SIFTS) (Q8TBC4) | Homo sapiens (human) | UBA3, Nedd8-activating enzyme hUba3 |
| 3 | E, F (E, F) | Ubiquitin-conjugating enzyme E2 M | polymer | 26 | 2875.4 | 2 | UniProt (P61081) | Homo sapiens (human) | UBC12N26, Ubiquitin-protein ligase M, Ubiquitin carrier protein M, Nedd8-conjugating enzyme Ubc12 |
| 4 | G, H (B, D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 5 | I, J, K, L, M (A, B, C, D, E) | water | water | 18.0 | 298 | Chemie (HOH) |
Sequence modifications
A, C: 1 - 534 (GenBank: 4502169)
B, D: 12 - 1013 (GenBank: 38045942)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | cloning artifact |
| Ser 0 | - | cloning artifact |
| - | Asn 254 | deletion |
| - | Glu 255 | deletion |
| - | Asn 256 | deletion |
| - | Gly 257 | deletion |
| - | Ala 258 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Met 9 | - | cloning artifact |
| Lys 10 | - | cloning artifact |
| Leu 11 | - | cloning artifact |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 223297.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 130.8 | |
| All* | Total formula weight | 223428.7 |
