1TK0
T7 DNA polymerase ternary complex with 8 oxo guanosine and ddCTP at the insertion site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | 5'-D(*CP*GP*AP*AP*AP*AP*CP*GP*A*CP*GP*GP*CP*CP*AP*GP*TP*GP*CP*CP*AP*(DDG))-3' | polymer | 22 | 6764.4 | 1 | |||
| 2 | B (T) | 5'-D(*CP*CP*CP*(8OG)P*CP*TP*GP*GP*CP*AP*CP*TP*GP*GP*CP*CP*GP*TP*CP*GP*TP*TP*TP*TP*CP*G)-3' | polymer | 26 | 7939.1 | 1 | |||
| 3 | C (A) | DNA polymerase | polymer | 698 | 79089.8 | 1 | UniProt (P00581) Pfam (PF00476) | Enterobacteria phage T7 | T7 DNA polymerase |
| 4 | D (B) | Thioredoxin 1 | polymer | 108 | 11687.4 | 1 | UniProt (P0AA25) Pfam (PF00085) | Escherichia coli | TRX1, TRX |
| 5 | E, F, G (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 6 | H, I (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 7 | J (A) | 2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE | non-polymer | 451.2 | 1 | Chemie (DCT) | |||
| 8 | K (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 9 | L (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 10 | M, N, O, P (P, T, A, B) | water | water | 18.0 | 533 | Chemie (HOH) |
Sequence modifications
A: 1 - 704 (UniProt: P00581)
| PDB | External Database | Details |
|---|---|---|
| - | Lys 118 | deletion |
| - | Arg 119 | deletion |
| - | Phe 120 | deletion |
| - | Gly 121 | deletion |
| - | Ser 122 | deletion |
| - | His 123 | deletion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 105480.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1149.7 | |
| All* | Total formula weight | 106630.4 |
