1SX8

EcoRV bound to cognate DNA and Mn2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	5'-D(*C*AP*AP*GP*AP*TP*AP*TP*CP*TP*T)-3'	polymer	11	3332.2	2
2	C, D (A, B)	Type II restriction enzyme EcoRV	polymer	244	28501.1	2	UniProt (P04390) Pfam (PF09233)	Escherichia coli	Endonuclease EcoRV, R.EcoRV
3	E, F, G, H, I... (A, B)	MANGANESE (II) ION	non-polymer		54.9	6	Chemie (MN)
4	K, L, M, N (C, D, A, B)	water	water		18.0	152	Chemie (HOH)

Sequence modifications

A, B: 2 - 245 (UniProt: P04390)

PDB	External Database	Details
Ala 92	Lys 91	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	63666.5
Non-Polymers*	Number of molecules	6
	Total formula weight	329.6
All*	Total formula weight	63996.2

*Water molecules are not included.