1SX8
EcoRV bound to cognate DNA and Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 |
Unit cell lengths | 47.800, 49.100, 63.700 |
Unit cell angles | 96.90, 108.90, 107.10 |
Refinement procedure
Resolution | 20.000 - 2.150 |
Rwork | 0.213 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.910 |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.180 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.089 | 0.307 |
Number of reflections | 23851 | |
<I/σ(I)> | 3.8 | 2 |
Completeness [%] | 0.9 | 89.7 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | PEG 4000, 0.1M HEPES, 0.15M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 100K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 2 | HEPES | ||
3 | 1 | 3 | NaCl |