1SUZ
The structure of K92A EcoRV bound to cognate DNA and Mg2+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (C, D) | 5'-D(*C*AP*AP*GP*AP*TP*AP*TP*CP*TP*T)-3' | polymer | 11 | 3332.2 | 2 | |||
| 2 | C, D (A, B) | Type II restriction enzyme EcoRV | polymer | 244 | 28501.1 | 2 | UniProt (P04390) Pfam (PF09233) | Escherichia coli | Endonuclease EcoRV, R.EcoRV |
| 3 | E, G (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | F, H (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 5 | I, J, K, L (C, D, A, B) | water | water | 18.0 | 303 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 245 (UniProt: P04390)
| PDB | External Database | Details |
|---|---|---|
| Ala 92 | Lys 91 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 63666.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 94.6 | |
| All* | Total formula weight | 63761.1 |
