1SUZ
The structure of K92A EcoRV bound to cognate DNA and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.410, 48.790, 63.400 |
| Unit cell angles | 97.10, 108.80, 107.30 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.197 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.037 | 0.324 |
| Number of reflections | 43796 | |
| <I/σ(I)> | 2.3 | 2 |
| Completeness [%] | 94.8 | 90.1 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 295 | 17% PEG 4K, 0.1M tartrate, 0.1M HPETES, 50mM MgCl2, pH 7.5, VAPOR DIFFUSION, temperature 22K |
