1SPB
SUBTILISIN BPN' PROSEGMENT (77 RESIDUES) COMPLEXED WITH A MUTANT SUBTILISIN BPN' (266 RESIDUES). CRYSTAL PH 4.6. CRYSTALLIZATION TEMPERATURE 20 C DIFFRACTION TEMPERATURE-160 C
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | P | SUBTILISIN BPN' PROSEGMENT | polymer | 78 | 8523.7 | 1 | UniProt (P00782) Pfam (PF05922) In PDB | Bacillus amyloliquefaciens | SUBTILISIN BPN' PROPEPTIDE |
2 | S | SUBTILISIN BPN' | polymer | 266 | 26620.5 | 1 | UniProt (P00782) Pfam (PF00082) In PDB | Bacillus amyloliquefaciens | |
3 | S | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
4 | water | water | 18.0 | 250 | Chemie (HOH) |
Sequence modifications
S: 1 - 275 (UniProt: P00782)
PDB | External Database | Details |
---|---|---|
Asn 32 | Asp 139 | engineered mutation |
Asn 43 | Lys 150 | engineered mutation |
Phe 50 | Met 157 | engineered mutation |
Leu 73 | Ala 180 | engineered mutation |
- | Leu 182 | deletion |
- | Asn 183 | deletion |
- | Asn 184 | deletion |
- | Ser 185 | deletion |
- | Ile 186 | deletion |
- | Gly 187 | deletion |
- | Val 188 | deletion |
- | Leu 189 | deletion |
- | Gly 190 | deletion |
Val 206 | Gln 313 | engineered mutation |
Lys 217 | Tyr 324 | engineered mutation |
Ser 218 | Asn 325 | engineered mutation |
Ala 221 | Ser 328 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 35144.2 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 23.0 | |
All* | Total formula weight | 35167.2 |