1SPB
SUBTILISIN BPN' PROSEGMENT (77 RESIDUES) COMPLEXED WITH A MUTANT SUBTILISIN BPN' (266 RESIDUES). CRYSTAL PH 4.6. CRYSTALLIZATION TEMPERATURE 20 C DIFFRACTION TEMPERATURE-160 C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | SUBTILISIN BPN' PROSEGMENT | polymer | 78 | 8523.7 | 1 | UniProt (P00782) Pfam (PF22148) | Bacillus amyloliquefaciens | SUBTILISIN BPN' PROPEPTIDE |
| 2 | B (S) | SUBTILISIN BPN' | polymer | 266 | 26620.5 | 1 | UniProt (P00782) Pfam (PF00082) | Bacillus amyloliquefaciens | |
| 3 | C (S) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 4 | D, E (P, S) | water | water | 18.0 | 250 | Chemie (HOH) |
Sequence modifications
S: 1 - 275 (UniProt: P00782)
| PDB | External Database | Details |
|---|---|---|
| Asn 32 | Asp 139 | engineered mutation |
| Asn 43 | Lys 150 | engineered mutation |
| Phe 50 | Met 157 | engineered mutation |
| Leu 73 | Ala 180 | engineered mutation |
| - | Leu 182 | deletion |
| - | Asn 183 | deletion |
| - | Asn 184 | deletion |
| - | Ser 185 | deletion |
| - | Ile 186 | deletion |
| - | Gly 187 | deletion |
| - | Val 188 | deletion |
| - | Leu 189 | deletion |
| - | Gly 190 | deletion |
| Val 206 | Gln 313 | engineered mutation |
| Lys 217 | Tyr 324 | engineered mutation |
| Ser 218 | Asn 325 | engineered mutation |
| Ala 221 | Ser 328 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 35144.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 23.0 | |
| All* | Total formula weight | 35167.2 |
