1SPB

SUBTILISIN BPN' PROSEGMENT (77 RESIDUES) COMPLEXED WITH A MUTANT SUBTILISIN BPN' (266 RESIDUES). CRYSTAL PH 4.6. CRYSTALLIZATION TEMPERATURE 20 C DIFFRACTION TEMPERATURE-160 C

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	113
Detector technology	CCD
Collection date	1994-06-21
Spacegroup name	P 21 21 2
Unit cell lengths	74.100, 77.850, 57.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.26 *
RMSD bond length	0.022
RMSD bond angle	0.046
Data reduction software	XENGEN
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.080
Number of reflections	17956
Completeness [%]	84.0
Redundancy	4.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulphate	2.0 (M)
2	1	reservoir	sodium acetate	0.1 (M)