1S0V
Structural basis for substrate selection by T7 RNA polymerase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | E, H, K, N | 5'-D(*G*GP*GP*AP*AP*TP*CP*GP*AP*TP*AP*TP*CP*GP*CP*CP*GP*C)-3' | polymer | 18 | 5541.6 | 4 | |||
| 2 | F, I, L, O | 5'-R(*AP*AP*CP*U*GP*CP*GP*GP*CP*GP*AP*U)-3' | polymer | 12 | 3851.4 | 4 | |||
| 3 | G, J, M, P | 5'-D(*GP*TP*CP*GP*AP*TP*TP*CP*CP*C)-3' | polymer | 10 | 2996.0 | 4 | |||
| 4 | A, B, C, D | DNA-directed RNA polymerase | polymer | 883 | 98984.2 | 4 | UniProt (P00573) Pfam (PF14700) Pfam (PF00940) In PDB | Enterobacteria phage T7 | |
| 5 | D, F, A, B, C | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
| 6 | D, A, B, C | DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER | non-polymer | 505.2 | 4 | Chemie (APC) | |||
| 7 | water | water | 18.0 | 999 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 883 (UniProt: P00573)
| PDB | External Database | Details |
|---|---|---|
| Leu 497 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 445492.6 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2215.3 | |
| All* | Total formula weight | 447707.9 |
