1S0V

Structural basis for substrate selection by T7 RNA polymerase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0006351	biological_process	DNA-templated transcription
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0006351	biological_process	DNA-templated transcription
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0006351	biological_process	DNA-templated transcription
D	0003677	molecular_function	DNA binding
D	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
D	0006351	biological_process	DNA-templated transcription

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG A 3001

Chain	Residue
A	APC2000
A	HOH3172
A	HOH3217

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site_id	AC2
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG B 3002

Chain	Residue
B	ASP471
B	APC2001

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site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG C 3003

Chain	Residue
C	APC2002
C	HOH3136

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site_id	AC4
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG D 3004

Chain	Residue
D	ASP471
D	APC2003

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site_id	AC5
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG F 3005

Chain	Residue
A	TYR639
A	APC2000
A	HOH3015
F	U8

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site_id	AC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG B 3006

Chain	Residue
B	TYR639
B	APC2001
B	HOH3164

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site_id	AC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MG C 3007

Chain	Residue
C	TYR639
C	APC2002

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site_id	AC8
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MG D 3008

Chain	Residue
D	TYR639
D	APC2003
O	U8

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site_id	AC9
Number of Residues	16
Details	BINDING SITE FOR RESIDUE APC A 2000

Chain	Residue
A	LYS472
A	TYR571
A	ARG627
A	LYS631
A	ARG632
A	MET635
A	THR636
A	MG3001
A	HOH3005
A	HOH3015
A	HOH3048
A	HOH3217
A	HOH3238
E	DT10
F	U8
F	MG3005

---

site_id	BC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE APC B 2001

Chain	Residue
B	LYS472
B	ARG627
B	LYS631
B	ARG632
B	MET635
B	MG3002
B	MG3006
B	HOH3164
B	HOH3218
H	DT10
H	DA11
I	U8

---

site_id	BC2
Number of Residues	15
Details	BINDING SITE FOR RESIDUE APC C 2002

Chain	Residue
C	TYR571
C	ARG627
C	LYS631
C	ARG632
C	MET635
C	THR636
C	MG3003
C	MG3007
C	HOH3136
C	HOH3152
C	HOH3154
C	HOH3207
C	HOH3208
K	DA11
L	U8

---

site_id	BC3
Number of Residues	12
Details	BINDING SITE FOR RESIDUE APC D 2003

Chain	Residue
D	LYS472
D	TYR571
D	ARG627
D	LYS631
D	ARG632
D	MET635
D	THR636
D	MG3004
D	MG3008
D	HOH3045
N	DT10
O	U8

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Functional Information from PROSITE/UniProt

site_id	PS00489
Number of Residues	15
Details	RNA_POL_PHAGE_2 Bacteriophage-type RNA polymerase family active site signature 2. VtRsvtKrsVMTlaY

Chain	Residue	Details
A	VAL625-TYR639

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site_id	PS00900
Number of Residues	12
Details	RNA_POL_PHAGE_1 Bacteriophage-type RNA polymerase family active site signature 1. PLafDGSCSGIQ

Chain	Residue	Details
A	PRO533-GLN544

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:8133519

Chain	Residue	Details
A	ASP537
A	ASP812
B	ASP537
B	ASP812
C	ASP537
C	ASP812
D	ASP537
D	ASP812

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site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: ACT_SITE => ECO:0000269|PubMed:1847871, ECO:0000269|PubMed:8133519

Chain	Residue	Details
A	LYS631
B	LYS631
C	LYS631
D	LYS631

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