1S0I
Trypanosoma cruzi trans-sialidase in complex with sialyl-lactose (Michaelis complex)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | trans-sialidase | polymer | 648 | 71345.3 | 1 | UniProt (Q26966) Pfam (PF13859) Pfam (PF22925) In PDB | Trypanosoma cruzi | |
| 2 | B | N-acetyl-alpha-neuraminic acid-(2-3)-beta-D-galactopyranose-(1-4)-alpha-D-glucopyranose | branched | 633.6 | 1 | In PDB BIRD (PRD_900025) GlyTouCan (G92323ZH) | 3'-sialyl-alpha-lactose | ||
| 3 | water | water | 18.0 | 590 | Chemie (HOH) |
Sequence modifications
A: 1 - 634 (UniProt: Q26966)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | cloning artifact |
| Gly -12 | - | cloning artifact |
| Gly -11 | - | cloning artifact |
| Ser -10 | - | cloning artifact |
| His -9 | - | cloning artifact |
| His -8 | - | cloning artifact |
| His -7 | - | cloning artifact |
| His -6 | - | cloning artifact |
| His -5 | - | cloning artifact |
| His -4 | - | cloning artifact |
| Gly -3 | - | cloning artifact |
| Met -2 | - | cloning artifact |
| Ala -1 | - | cloning artifact |
| Ser 0 | - | cloning artifact |
| Phe 58 | Asn 59 | engineered mutation |
| Ala 59 | Asp 60 | engineered mutation |
| Thr 262 | Ser 263 | SEE REMARK 999 |
| His 476 | Arg 477 | SEE REMARK 999 |
| Leu 484 | Val 485 | SEE REMARK 999 |
| Lys 495 | Ser 496 | engineered mutation |
| Gly 496 | Val 497 | engineered mutation |
| Lys 520 | Glu 521 | engineered mutation |
| Val 558 | Glu 559 | SEE REMARK 999 |
| Gly 593 | Asp 594 | engineered mutation |
| Asp 597 | Ile 598 | engineered mutation |
| Arg 599 | His 600 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 71345.3 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 633.6 | |
| All* | Total formula weight | 71978.8 |
