1S0I
Trypanosoma cruzi trans-sialidase in complex with sialyl-lactose (Michaelis complex)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2002-09-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9330 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.296, 129.443, 54.316 |
| Unit cell angles | 90.00, 107.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.16475 |
| Rwork | 0.164 |
| R-free | 0.18704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ms1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.616 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.034 | 0.114 |
| Number of reflections | 90100 | |
| Completeness [%] | 96.2 | 80.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, Tris-HCl, isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
