1S01
LARGE INCREASES IN GENERAL STABILITY FOR SUBTILISIN BPN(PRIME) THROUGH INCREMENTAL CHANGES IN THE FREE ENERGY OF UNFOLDING
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Subtilisin BPN' | polymer | 275 | 27543.6 | 1 | UniProt (P00782) Pfam (PF00082) | Bacillus amyloliquefaciens (Bacillus velezensis) | Alkaline protease,Subtilisin DFE,Subtilisin Novo |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C, D (A) | ISOPROPYL ALCOHOL | non-polymer | 60.1 | 2 | Chemie (IPA) | |||
| 4 | E (A) | water | water | 18.0 | 215 | Chemie (HOH) |
Sequence modifications
A: 1 - 275 (UniProt: P00782)
| PDB | External Database | Details |
|---|---|---|
| Phe 50 | Met 157 | engineered mutation |
| Asp 76 | Asn 183 | engineered mutation |
| Ala 169 | Gly 276 | engineered mutation |
| Sch 206 | Gln 313 | engineered mutation |
| Lys 217 | Tyr 324 | engineered mutation |
| Ser 218 | Asn 325 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27543.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 160.3 | |
| All* | Total formula weight | 27703.9 |
