1RQQ

Crystal Structure of the Insulin Receptor Kinase in Complex with the SH2 Domain of APS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Insulin receptor	polymer	306	35033.7	2	UniProt (P06213) Pfam (PF07714) In PDB	Homo sapiens (human)	IR
2	C, D	adaptor protein APS	polymer	114	12891.7	2	UniProt (Q9Z200) Pfam (PF00017) In PDB	Rattus norvegicus (Norway rat)
3	E, F	BISUBSTRATE INHIBITOR	polymer	18	1939.3	2
4	A, B	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
5	E, F	THIOPHOSPHORIC ACID O-((ADENOSYL-PHOSPHO)PHOSPHO)-S-ACETAMIDYL-DIESTER	non-polymer		580.3	2	Chemie (112)
6		water	water		18.0	79	Chemie (HOH)

Sequence modifications

A, B: 978 - 1283 (UniProt: P06213)

PDB	External Database	Details
Ser 981	Cys 1008	engineered mutation
Ptr 1158	Tyr 1185	modified residue
Ptr 1162	Tyr 1189	modified residue
Ptr 1163	Tyr 1190	modified residue
Asn 1251	Lys 1278	variant

C, D: 401 - 510 (UniProt: Q9Z200)

PDB	External Database	Details
Gly 397	-	expression tag
Ser 398	-	expression tag
His 399	-	expression tag
Met 400	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	99729.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1270.5
All*	Total formula weight	100999.7

*Water molecules are not included.