1QS0

Crystal Structure of Pseudomonas Putida 2-oxoisovalerate Dehydrogenase (Branched-Chain Alpha-Keto Acid Dehydrogenase, E1B)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	2-OXOISOVALERATE DEHYDROGENASE ALPHA-SUBUNIT	polymer	407	45594.4	1	UniProt (P09060) Pfam (PF12573) Pfam (PF00676) In PDB	Pseudomonas putida
2	B	2-OXOISOVALERATE DEHYDROGENASE BETA-SUBUNIT	polymer	338	37509.9	1	UniProt (P09061) Pfam (PF02779) Pfam (PF02780) In PDB	Pseudomonas putida
3	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	A	THIAMINE DIPHOSPHATE	non-polymer		425.3	1	Chemie (TPP)
5	B	2-OXO-4-METHYLPENTANOIC ACID	non-polymer		130.1	1	Chemie (COI)
6		water	water		18.0	197	Chemie (HOH)

Sequence modifications

A: 2 - 408 (UniProt: P09060)

PDB	External Database	Details
Mse 81	Met 81	modified residue
Mse 84	Met 84	modified residue
Mse 94	Met 94	modified residue
Mse 103	Met 103	modified residue
Mse 107	Met 107	modified residue
Mse 128	Met 128	modified residue
Mse 140	Met 140	modified residue
Mse 149	Met 149	modified residue
Mse 169	Met 169	modified residue
Mse 196	Met 196	modified residue
Mse 389	Met 389	modified residue
Mse 397	Met 397	modified residue

B: 2 - 339 (UniProt: P09061)

PDB	External Database	Details
Mse 6	Met 6	modified residue
Mse 8	Met 8	modified residue
Mse 16	Met 16	modified residue
Mse 19	Met 19	modified residue
Mse 72	Met 72	modified residue
Mse 100	Met 100	modified residue
Mse 119	Met 119	modified residue
Mse 138	Met 138	modified residue
Mse 149	Met 149	modified residue
Mse 337	Met 337	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83104.3
Non-Polymers*	Number of molecules	3
	Total formula weight	579.8
All*	Total formula weight	83684.0

*Water molecules are not included.