1QS0
Crystal Structure of Pseudomonas Putida 2-oxoisovalerate Dehydrogenase (Branched-Chain Alpha-Keto Acid Dehydrogenase, E1B)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Detector technology | IMAGE PLATE |
Collection date | 1998-06-21 |
Detector | MARRESEARCH |
Wavelength(s) | 0.88561,0.97888,0.97894 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 101.340, 101.340, 381.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.400 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.26500 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | SHARP |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.000 | |
High resolution limit [Å] | 2.400 | |
Rmerge | 0.077 | 0.182 * |
Total number of observations | 407418 * | |
Number of reflections | 56728 * | |
<I/σ(I)> | 16 | |
Completeness [%] | 97.3 | 74 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 295 | POTASSIUM PHOSPHATE, DTT, MAGNESIUM CHLORIDE, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 50 (mM) | |
3 | 1 | drop | ThDP | 1 (mM) | |
4 | 1 | drop | 4 (mM) | ||
5 | 1 | drop | L-valine | 10 (mM) | |
6 | 1 | drop | dithiothreitol | 4-12 (mM) | |
7 | 1 | reservoir | sodium potassium phosphate | 1.8-2.5 (M) | |
8 | 1 | reservoir | 0.01 (%) | ||
9 | 1 | reservoir | dithiothreitol | 4-12 (mM) |