1PVR

BASIS FOR A SWITCH IN SUBSTRATE SPECIFICITY: CRYSTAL STRUCTURE OF SELECTED VARIANT OF CRE SITE-SPECIFIC RECOMBINASE, LNSGG BOUND TO THE LOXP (WILDTYPE) RECOGNITION SITE

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	34-MER	polymer	34	10470.8	1	GenBank (215623)
2	B (D)	34-MER	polymer	34	10439.8	1	GenBank (215626)
3	C, D (A, B)	Recombinase CRE	polymer	349	39222.8	2	UniProt (P06956) Pfam (PF00589)	Enterobacteria phage P1
4	E, F, G, H (C, D, A, B)	water	water		18.0	103	Chemie (HOH)

Sequence modifications

A, B: 2 - 343 (UniProt: P06956)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Leu 174	Ile 174	engineered mutation
Asn 258	Thr 258	engineered mutation
Ser 259	Arg 259	engineered mutation
Gly 262	Glu 262	engineered mutation
Gly 266	Glu 266	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	99356.2
All*	Total formula weight	99356.2

*Water molecules are not included.