1P7T

Structure of Escherichia coli malate synthase G:pyruvate:acetyl-Coenzyme A abortive ternary complex at 1.95 angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Malate synthase G	polymer	731	81652.5	1	UniProt (P37330) Pfam (PF20656) Pfam (PF20658) Pfam (PF01274) Pfam (PF20659) In PDB	Escherichia coli str. K12 substr.	MSG
2	B	Malate synthase G	polymer	731	81668.5	1	UniProt (P37330) Pfam (PF20656) Pfam (PF20658) Pfam (PF01274) Pfam (PF20659) In PDB	Escherichia coli str. K12 substr.	MSG
3	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	A, B	ACETYL COENZYME *A	non-polymer		809.6	2	Chemie (ACO)
5	A, B	PYRUVIC ACID	non-polymer		88.1	2	Chemie (PYR)
6	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
7	B	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
8		water	water		18.0	583	Chemie (HOH)

Sequence modifications

A: 2 - 723 (UniProt: P37330)

PDB	External Database	Details
Met 1	-	see remark 999
Ala 2	Ser 1	cloning artifact
Cso 617	Cys 616	modified residue
Leu 724	-	expression tag
Glu 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag
His 730	-	expression tag
His 731	-	expression tag

B: 2 - 723 (UniProt: P37330)

PDB	External Database	Details
Met 1	-	see remark 999
Ala 2	Ser 1	cloning artifact
Cso 617	Cys 616	modified residue
Cso 688	Cys 687	modified residue
Leu 724	-	expression tag
Glu 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag
His 730	-	expression tag
His 731	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	163321.0
Non-Polymers*	Number of molecules	9
	Total formula weight	2338.4
All*	Total formula weight	165659.5

*Water molecules are not included.