1P7K
Crystal structure of an anti-ssDNA antigen-binding fragment (Fab) bound to 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (L, A) | antibody light chain FAB | polymer | 214 | 23616.1 | 2 | UniProt (Q8VCP0) Pfam (PF07654) UniProt (by SIFTS) (P01837) | Mus musculus (house mouse) | |
| 2 | B, D (H, B) | antibody heavy chain FAB | polymer | 224 | 24153.2 | 2 | UniProt (Q9JL75) Pfam (PF07654) UniProt (by SIFTS) (P01868) | Mus musculus (house mouse) | |
| 3 | CA, DA, E, N, O... (B, L, H, A) | SULFATE ION | non-polymer | 96.1 | 9 | Chemie (SO4) | |||
| 4 | EA, F (B, L) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 2 | Chemie (EPE) | |||
| 5 | FA, G, GA, S (B, L, H) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 4 | Chemie (1PE) | |||
| 6 | H, T (L, H) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 2 | Chemie (PEG) | |||
| 7 | AA, BA, HA, I, IA... (A, B, L, H) | GLYCEROL | non-polymer | 92.1 | 16 | Chemie (GOL) | |||
| 8 | LA, MA, NA, OA (L, H, A, B) | water | water | 18.0 | 771 | Chemie (HOH) |
Sequence modifications
H, B: 5 - 217 (UniProt: Q9JL75)
| PDB | External Database | Details |
|---|---|---|
| Gln 1 | - | cloning artifact |
| Val 2 | - | cloning artifact |
| Lys 3 | - | cloning artifact |
| Leu 4 | - | cloning artifact |
| Pro 52 | - | insertion |
| Ser 82 | - | insertion |
| Ser 82 | - | insertion |
| Leu 82 | - | insertion |
| Tyr 100 | - | insertion |
| Ala 100 | - | insertion |
| Met 100 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 95538.4 | |
| Non-Polymers* | Number of molecules | 33 |
| Total formula weight | 3980.0 | |
| All* | Total formula weight | 99518.5 |
