1OT2

Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135N

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cyclomaltodextrin glucanotransferase	polymer	686	74558.4	1	UniProt (P43379) Pfam (PF00128) Pfam (PF02806) Pfam (PF01833) Pfam (PF00686)	Bacillus circulans	Cyclodextrin-glycosyltransferase, CGTase
2	B (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		1153.0	1	In PDB GlyTouCan (G99976JK)
3	C, D (C, D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		666.6	2	In PDB GlyTouCan (G69211UR)
4	E (E)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
5	F, H, I (A)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
6	G (A)	alpha-D-glucopyranose	non-polymer		180.2	1	Chemie (GLC)
7	J, O, P (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	3	Chemie (MPD)
8	K, L, M (A)	ACETIC ACID	non-polymer		60.1	3	Chemie (ACY)
9	N (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
10	Q (A)	water	water		18.0	907	Chemie (HOH)

Sequence modifications

A: 1 - 686 (UniProt: P43379)

PDB	External Database	Details
Asn 135	Asp 162	engineered mutation
Ser 400	Cys 427	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	74558.4
Branched	Number of molecules	4
	Total formula weight	2828.5
Non-Polymers*	Number of molecules	11
	Total formula weight	1073.4
All*	Total formula weight	78460.3

*Water molecules are not included.