1OT1
Bacillus circulans strain 251 Cyclodextrin glycosyl transferase mutant D135A
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Cyclomaltodextrin glucanotransferase | polymer | 686 | 74515.4 | 1 | UniProt (P43379) Pfam (PF00128) Pfam (PF02806) Pfam (PF01833) Pfam (PF00686) In PDB | Bacillus circulans | Cyclodextrin-glycosyltransferase, CGTase |
| 2 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 1153.0 | 1 | In PDB GlyTouCan (G99976JK) | |||
| 3 | C, D | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 666.6 | 2 | In PDB GlyTouCan (G69211UR) | |||
| 4 | E | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 5 | A | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
| 6 | A | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 3 | Chemie (MPD) | |||
| 7 | A | ACETIC ACID | non-polymer | 60.1 | 4 | Chemie (ACY) | |||
| 8 | A | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 1 | Chemie (EPE) | |||
| 9 | water | water | 18.0 | 824 | Chemie (HOH) |
Sequence modifications
A: 1 - 686 (UniProt: P43379)
| PDB | External Database | Details |
|---|---|---|
| Ala 135 | Asp 162 | engineered mutation |
| Ser 400 | Cys 427 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 74515.4 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2828.5 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 953.3 | |
| All* | Total formula weight | 78297.1 |
