1O5Q

Crystal Structure of Pyruvate and Mg2+ bound 2-methylisocitrate lyase (PrpB) from Salmonella typhimurium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Probable methylisocitrate lyase	polymer	305	33264.8	4	UniProt (Q56062) Pfam (PF13714)	Salmonella enterica subsp. enterica serovar Typhimurium	2-methylisocitrate lyase
2	E, G, I, K (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
3	F, H, J, L (A, B, C, D)	PYRUVIC ACID	non-polymer		88.1	4	Chemie (PYR)
4	M, N, O, P (A, B, C, D)	water	water		18.0	553	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 295 (UniProt: Q56062)

PDB	External Database	Details
Met -1	-	cloning artifact
Ala 0	-	cloning artifact
Ser 1	-	cloning artifact
Leu 296	-	expression tag
Glu 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag
His 302	-	expression tag
His 303	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	133059.3
Non-Polymers*	Number of molecules	8
	Total formula weight	449.5
All*	Total formula weight	133508.8

*Water molecules are not included.