1NMM

beta-1,4-galactosyltransferase mutant Cys342Thr complex with alpha-lactalbumin and GlcNAc

Replaces:  1JN8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	ALPHA-LACTALBUMIN	polymer	123	14015.8	2	UniProt (P29752) Pfam (PF00062)	Mus musculus (house mouse)	lactalbumin, alpha
2	B, D (B, D)	BETA-1,4-GALACTOSYLTRANSFERASE	polymer	286	32849.6	2	UniProt (P08037) Pfam (PF13733) Pfam (PF02709)	Bos taurus (cattle)	B4gal-t1; BETA4GAL-T1; BETA-1,4-GALTASE 1; BETA-1,4-GALACTOSYLTRANSFERASE 1; UDP-GALACTOSE:BETA-N-ACETYLGLUCOSAMINE BETA-1,4-GALACTOSYLTRANSFERASE 1
3	E, G (A, C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	F, H (B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
5	I, J (D)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
6	K, L, M, N (A, B, C, D)	water	water		18.0	782	Chemie (HOH)

Sequence modifications

B, D: 130 - 402 (UniProt: P08037)

PDB	External Database	Details
Ala 117	-	SEE REMARK 999
Ser 118	-	SEE REMARK 999
Met 119	-	SEE REMARK 999
Thr 120	-	SEE REMARK 999
Gly 121	-	SEE REMARK 999
Gly 122	-	SEE REMARK 999
Gln 123	-	SEE REMARK 999
Gln 124	-	SEE REMARK 999
Met 125	-	SEE REMARK 999
Gly 126	-	SEE REMARK 999
Arg 127	-	SEE REMARK 999
Gly 128	-	SEE REMARK 999
Ser 129	-	SEE REMARK 999
Thr 342	Cys 269	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	93730.8
Non-Polymers*	Number of molecules	6
	Total formula weight	911.0
All*	Total formula weight	94641.9

*Water molecules are not included.