1NMM
beta-1,4-galactosyltransferase mutant Cys342Thr complex with alpha-lactalbumin and GlcNAc
Replaces: 1JN8Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9B |
| Synchrotron site | NSLS |
| Beamline | X9B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.019, 95.626, 100.213 |
| Unit cell angles | 90.00, 101.37, 90.00 |
Refinement procedure
| Resolution | 24.720 - 2.000 |
| R-factor | 0.1891 |
| Rwork | 0.189 |
| R-free | 0.30000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.079 * | |
| Total number of observations | 265383 * | |
| Number of reflections | 71567 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 298 | PEG 4000, Sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Gal-T1 | 20 (mg/ml) | |
| 2 | 1 | drop | LA | 10 (mg/ml) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | pH5.6 |
| 4 | 1 | reservoir | PEG4000 | 12.5 (%) | |
| 5 | 1 | reservoir | 100 (mM) |
