1N4A

The Ligand Bound Structure of E.coli BtuF, the Periplasmic Binding Protein for Vitamin B12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Vitamin B12 transport protein btuF	polymer	252	28140.7	2	UniProt (P37028) Pfam (PF01497)	Escherichia coli
2	C, D (A, B)	CYANOCOBALAMIN	non-polymer		1356.4	2	Chemie (CNC)
3	E, F (A, B)	water	water		18.0	549	Chemie (HOH)

Sequence modifications

A, B: 1 - 244 (UniProt: P37028)

PDB	External Database	Details
Mse 47	Met 69	modified residue
Mse 83	Met 105	modified residue
Leu 245	-	expression tag
Glu 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
His 252	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56281.4
Non-Polymers*	Number of molecules	2
	Total formula weight	2712.7
All*	Total formula weight	58994.1

*Water molecules are not included.